1234567890abcdef

with open("test.txt") as fin,  open("test.bin", "wb") as fout:
    s = fin.read()
    fout.write(int(s,16).to_bytes(len(s)//2,"big"))

import re
with open('test.txt', 'r') as fin:
  s = fin.read()
  s = re.sub(r'[\r\n\t ]|0x', '', s)
  bins = [int(a+b,16) for a, b in zip(s[::2], s[1::2])]
  with open('test.bin', 'wb') as fout:
     fout.write(bytearray(bins))

In [59]: s = "1234567890abcdef"
    ...: for a, b in zip(s[::2], s[1::2]):
    ...:     print(f"a={a}, b={b}")
a=1, b=2
a=3, b=4
a=5, b=6
a=7, b=8
a=9, b=0
a=a, b=b
a=c, b=d
a=e, b=f

0x12 0x34 0x56 0x78 0x90

1234
56789
abc
def

import codecs
with open('test.txt', 'r') as fin, open('test.bin', 'wb') as fout:
  s = re.sub(r'[\r\n\t ]|0x', '', fin.read())
  fout.write(bytearray(codecs.getdecoder("hex_codec")(s)[0])

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